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KMID : 0380020060210040298
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 4 p.298 ~ p.301
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Production of Recombinant Trehalose Synthase from Thermus caldophilus GK24
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Choi Jae-Youl
Cha Wol-Suk
Shin Hyun-Jae
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Abstract
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A gene (GeneBank AF 135796) coding for a trehalose synthase from Thermus caldophilus GK24 was cloned into Escherichia coli K12 using five vector systems. The constitutive expression system (pHCETS) which shows the highest trehalose synthase activity from flask culture of recombinant E. coli was selected for the production of trehalose from maltose. For the shake flask culture, the final dry cell weight was 0.9 g/L and the trehalose synthase activity was 25 U/mL. Fed-batch culture of recombinant E. coli harboring plasmid pHCETS which uses the glycerolas a carbon source was performed in jar fermentor: the dry cell weight of 20 g/L and the trehalose synthase activity of 13.7 U/mL were attained in 48 h.
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KEYWORD
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Trehalose, trehalose synthase, Thermus caldophilus GK24, constitutive expression vector, fed-batch culture
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